Simultaneous determination of eight flavonoids in Polygonum aviculare L. by RPHPLCUV

A reliable high performance liquid chromatography method was developed for the quality evaluation of Polygonum aviculare L. Eight marker flavonoids were identified and simultaneously quantified, which included myricitrin, hyperoside, galuteolin, avicularin, quercitrin, quercetin, luteolin, and kaempferol. The analysis was performed on an Inertsil ODS4 column (4.6 mm×150 mm, 5 μm) with gradient elution. The mobile phases were 0.5% aqueous phosphoric acid and acetonitrile. The detection wavelength was 360 nm. The eight marker flavonoids were separated well with good linearity (r~2 >0.9991), precision, stability and repeatability. The recovery rate was 95.58%–102.65%. Cluster analysis was employed to analyze 28 batches of samples. The result indicated that this method provides an efficient way to perform quality control as well as a scientific rationale for the Geoauthentication of Polygonum aviculare L.Study of Nature of Geoauthentic Crude Drug (“973” State Key Project)中国科学引文数据库(CSCD)3170-1762