The involvement of signaling activation of protein kinase C in gadolinium chloride-induced cell survival and cell cycle progression in NIH3T3 cells

In the present study, we investigated the activation of protein kinase C (PKC) family in mouse embryonic fibroblast NIH3T3 cells using gadolinium chloride as a representative lanthanide ion. With live cell imaging system and confocal laser scanning microscopy, we found that the treatment of 50 μM GdCl_3 promoted cell survival under the condition of serum-starvation. Moreover, better cell attachment and cytoskeleton reorganization were also observed. Additionally, GdCl_3 treatment resulted in the phosphorylation of PKC family at different time points. Furthermore, bisindolylmaleimide (a PKCpan inhibitor) could efficiently reduce the level of phosphorylated PKCpan (βII~(Ser660)),alleviating ERK activation induced by GdCl_3. This finding indicated that the PKC activation was involved in GdCl_3-induced MAPK/ERK signaling and thus might contribute to GdCl_3-induced cell cycle progression and cell survival.National Natural Science Foundation of China中国科学引文数据库(CSCD)11772-7772