Chromium (VI) potentiates the DNA adducts (O-6-methylguanine) formation of N-nitrosodimethylamine in rat: Implication on carcinogenic risk

Chromium (VI) [Cr(VI)] and nitrosamines such as N-nitrosodimethylamine (NDMA) exist commonly in the environment. To evaluate the potential influence of Cr(VI) co-exposure on the carcinogenic risk of NDMA, Female Wistar rats were treated with various concentrations of Cr(VI) and/or NDMA via drinking water for 15 days and the DNA adducts (O-6-methylguanine, O-6-MeG) of NDMA in liver tissue was used as a bioindicator. The results showed that Cr(VI) synergistically enhanced the O-6-MeG formation, which could lead to an increase in DNA damage and carcinogenic potential. Although Cr(VI) did not alter the CYP 2E1 enzyme activity, it decreased GSH content, which would be an potential mechanism for the potentiated O-6-MeG formation by Cr(VI) co-exposure. These results would contribute to the development of quantitative risk assessment of NDMA or even for a group of nitrosamines under environmental mixture exposure. (C) 2015 Elsevier Ltd. All rights reserved.National Natural Science Foundation of China [41330637]; National Special Funding Project for Water Pollution Control and Management of China [2014ZX07405001]SCI(E)EIPubMedARTICLEhujy@urban.pku.edu.cn256-25913