Comprehensive identification of 125 multifarious constituents in Shuang-huang-lian powder injection by HPLC-DAD-ESI-IT-TOF-MS

Sun, Hongyang
Liu, Meixian
Lin, Zongtao
Jiang, Haixiu
Niu, Yanyan
Wang, Hong
Chen, Shizhong

Open link

Publication date

January 2015

DOI

10.1016/j.jpba.2015.06.013

Publisher

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

ISSN

0731-7085

Journal

issn:0731-7085

Citation count (estimate)

Abstract

A high-performance liquid chromatography-diode array detector-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) method was established for excellent separation and structural identification of constituents in Shuang-huang-lian powder injection (SHLPI). The typical ultraviolet absorptions, accurate empirical molecular formula and reasonable fragmentation mechanisms of these ingredients were used for their structural elucidation. In consequence, 125 constituents (33 phenolic acids, 29 flavonoids, 32 phenylethanoid glycosides, 15 iridoid glycosides, 8 lignans, 3 amino acids and 2 purines nucleosides, 2 quinoid glycosides and 1 alkylbenzene glycoside) were either unequivocally identified or tentatively c...