Spectroscopic research on the interaction of 2-(4-dimethylaminophenyl)-5-fluoro-6-(morpholin-4-yl) - 1H-benzimidazole and calf thymus DNA

UV-Vis spectra of the aqueous solutions of 2-(4-dimethylarninophenyl)-5-fluoro-6-(morpholin-4-yl) - 1H-benzimidazole (1) at different pH values revealed that compound 1 is a tertiary base, which can combine three protons. Through the non-linear least square method, the logarithm of the three accumulative protonation constants of compound 1, namely, lg??1, lg??2 and l lg??3, were determined to be 4.96 ?? 0.03, 5.72 ?? 0.07 and 7.95 ?? 0.10, respectively. UV-Vis and the steady-state fluorescence spectra indicated that a special interaction occurs between compound 1 and calf thymus DNA at the pH value of 3.40, of which the binding constant, Kb, is (2.30 ?? 0.10) ?? 104 mol-1??L. Compound 1 in the concentration range of 10-8 to 1.2 ?? 10-6 mol??L-1 has a potential application to the quantitative determination of DNA.SCI(E)EIPubMed中文核心期刊要目总览(PKU)中国科技核心期刊(ISTIC)中国科学引文数据库(CSCD)0101618-16212