Removal of Pentachlorophenol (PCP) by Immobilized Horseradish Peroxidase (HRP)

Researches on the removal of PCP by free HRP with the existence of H2O2 were conducted. PCP was measured by safranine-T spectrophotometric method (GB 9803-88). Results indicated that HRP could effectively oxidate PCP in aqueous solution. Factors, such as acidity, temperature, enzyme activity, and initial concentration of PCP and H2O2, which could influence the oxidation, were studied. Results showed that the optimum pH value for free enzyme was 5~6; relative higher temperature could accelerate the reaction greatly; removal rate increased with the increase of enzyme concentration, and removal rate could reach nearly 70% under the highest enzyme concentration (about 0.05 u/mL) adopted in the experiment; removal rate increased slightly with the increase of initial concentration of PCP; the molar ratio of the reaction between PCP and H2O2 is about 1 : 2. Based on above results, researches on the oxidation of PCP by immobilized HRP were conducted. Firstly, through chemical bonding method, free HRP was immobilized on polyacrylamide gel prepared by ?? ray radiation method; then immobilized HRP was filled into a column, and PCP was successfully oxidated by immobilized HRP column. Finally, results were analyzed and compared with results using free HRP.EI06918-9254