Large-capacity expanded cytoline-induced killer cells and its cytotoxic activity

This study was conducted to establish the large-capacity culture method of cytokine-induced killer (CIK) cells for clinical therapy and assess its effect on the function of cell-mediated immunity following autologous CIK cells reinfusion. Autologous CIK cells were expanded in 1000 ml culture-bag and reinfused back. The MTT method was used to test the cytotoxic activity of CIK cells before and after reinfusion. The results showed that the total amount of autologous CIK cells reinfusion exceeded 1.6 ?? 1010 with the use of the culture method of large-capacity. The PBMNC from patients treated by CIK cells showed significant increase in cytotoxic activity, no side effects were observed, and therefore the large-capacity culture method of CIK cells is a simple and safe therapy for treating the minimum residue of diseases.EI0194-961