We used a total internal reflection fluorescence microscope based on the near field optics principle to study the Ca2+signals in single heart cells of Sprague-Dawley rat. Due to the high signal to noise ratio and high speed of data acquisition of this microscope, the Ca2+ images show complex 2-dimensional wave patterns. The local elementary Ca2+ release units play important role in the formation and transformation of Ca2+ wave. Simulations based on Fire-Diffuse-Fire model show that the Ca2+ wave, composed of Ca2+ release units, may steadily exist in single heart cell. Our results will be helpful for understanding the micro-dynamics of living excitable medium.EI063607-36125
We describe a novel two-photon (2P) laser scanning microscopy (2PLSM) protocol that provides ratiome...
The elementary release of calcium (Ca2+) from a cell’s internal store is known as a Ca2+ spark. The ...
We measured [Ca2+]i of guinea pig and rat myocytes with Ca2+ waves, using fura-2 fluorescence image ...
利用基于近场光学原理构建的全内反射荧光显微镜研究了大鼠单个心肌细胞中的钙信号.利用这种显微镜的快速成像和高信噪比的特点,观察到单个细胞中复杂的二维钙波斑图.分析了单个钙信号释放事件在钙波形成、运动过程...
In cardiac research, single-cell experimental models have been extensively used to study the molecul...
Based on near-field optical theory, total internal reflection fluorescence microscope shows a novel ...
The regenerative Ca(2+)-induced Ca2+ release mechanism is an important amplifier of signal transduct...
Spontaneous calcium waves in enzymatically isolated rat cardiac myocytes were investigated by confoc...
AbstractA novel Nipkow-type confocal microscope was applied to image spontaneously propagating Ca2+ ...
The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `buildi...
Waves of concentration change of specific signal molecules play an important role in signal exchange...
<p>Background: Understanding the microscopic dynamics of the beating heart has been challenging due ...
Intracellular Ca2+ ([Ca2+]i) dynamics in isolated myocytes differ between the atria and ventricles d...
<p>Background: Understanding the microscopic dynamics of the beating heart has been challenging due ...
Objective: The aim of this study was to characterize the spatio-temporal dynamics of slow Ca2+ waves...
We describe a novel two-photon (2P) laser scanning microscopy (2PLSM) protocol that provides ratiome...
The elementary release of calcium (Ca2+) from a cell’s internal store is known as a Ca2+ spark. The ...
We measured [Ca2+]i of guinea pig and rat myocytes with Ca2+ waves, using fura-2 fluorescence image ...
利用基于近场光学原理构建的全内反射荧光显微镜研究了大鼠单个心肌细胞中的钙信号.利用这种显微镜的快速成像和高信噪比的特点,观察到单个细胞中复杂的二维钙波斑图.分析了单个钙信号释放事件在钙波形成、运动过程...
In cardiac research, single-cell experimental models have been extensively used to study the molecul...
Based on near-field optical theory, total internal reflection fluorescence microscope shows a novel ...
The regenerative Ca(2+)-induced Ca2+ release mechanism is an important amplifier of signal transduct...
Spontaneous calcium waves in enzymatically isolated rat cardiac myocytes were investigated by confoc...
AbstractA novel Nipkow-type confocal microscope was applied to image spontaneously propagating Ca2+ ...
The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `buildi...
Waves of concentration change of specific signal molecules play an important role in signal exchange...
<p>Background: Understanding the microscopic dynamics of the beating heart has been challenging due ...
Intracellular Ca2+ ([Ca2+]i) dynamics in isolated myocytes differ between the atria and ventricles d...
<p>Background: Understanding the microscopic dynamics of the beating heart has been challenging due ...
Objective: The aim of this study was to characterize the spatio-temporal dynamics of slow Ca2+ waves...
We describe a novel two-photon (2P) laser scanning microscopy (2PLSM) protocol that provides ratiome...
The elementary release of calcium (Ca2+) from a cell’s internal store is known as a Ca2+ spark. The ...
We measured [Ca2+]i of guinea pig and rat myocytes with Ca2+ waves, using fura-2 fluorescence image ...