Cre/loxP-mediated deletion of transgene in transgenic tobacco

In the present study, we develop an efficient method to be used to examine recombination efficiency of transgenic plantlet. Two constructs, one containing Cre recombinase under the control of 35S promoter (p35S/Cre) and the other containing a uidA gene driven by 35S promoter flanked by loxP site (loxP/35S/GUS/loxP), were simultaneously introduced into tobacco by cotransformation method. The results show that Cre-loxP system can precisely and efficiently direct deletion of transgene based on Gus assay, PCR analysis and sequence of recombination DNA fragment, but also find that incomplete deletion exist in partial plant.EI04477-4823