Accelerator Mass Spectrometry Studies on Adduction of Nicotine with Hemoglobin and Serum Albumin in Mouse

Adducts of hemoglobin (Hb) and serum albumin (SA) with nicotine in mouse blood were measured respectively by using accelerator mass spectrometry (AMS). The dose response of adduct levels of Hb/SA and the correlative relationship between the two blood protein adducts were investigated over a range of doses(0.1-100. 0 ??g/kg body weight), which were equivalent to a low-level dose of environmental exposure of cigarette smoking. Both the levels of Hb and SA adducts increased with increasing dose and showed a linear relationship. By plotting Hb/SA adducts versus DNA adducts, a linear relationship fits the experimental observations best, which means that Hb/SA adduct could substitute for DNA adduct as a biomarker for nicotine. The detection limit of protein adduct in this work is 0. 03 pmol/g protein (namely 3. 2 adducts/1012 amino acid residues), the excellent sensitivity in protein biomarker measurements compared to the data reported previously.EI05811-8122