Localization of specific glycoproteins of sperm cell membrane from Zea mays by electronmicroscopy immuno-gold labelling

Plasma membrane proteins of sperm cells from Zea mays were passed through affinity column, separated by SDS-PAGE and stained using Con A-HRP. The components of 37kD and 39kD glycoproteins from gel were immunized respectively mice and polyclonal antiserums were obtained. Glycoproteins of sperm cell surface can be examined using IgG(rabbit anti mouse)-colloidal gold(15nm) as probe. The results showed that distribution of 37 and 39kD peptides on the plasma membrane surface of sperm cells of maize with visible immunogold particles to be localized. We consider that 37 and 39kD polypeptide were specific plasma membrane glycopeptides of sperm cells. Research of their function is to be continued.EI02193-1973