Isolation and identification of isoflavones from trifolium pratense

To investigate the chemical constituents from the ethanolic extract of Trifolium pratense. Eight known compounds were isolated by using various column chromatographies and identified on the basis of spectral analysis. These compounds are prunetin (1), irilone (2), biochanin A (3), formononetin (4), pratensein (5), genistein (6), calycosin (7) and daidzein (8).EI04544-5494