Biological Effects of Osteoblast-Like Cells on Nanohydroxyapatite Particles at a Low Concentration Range

The biological effects of osteoblast-like MG-63 cells on nanohydroxyapatite (n-HA) at the low concentration range (5-25 mu g/mL) for 5 days was investigated. The results showed the viability and actin cytoskeleton of the cells descended with the increase of the concentration of n-HA, and the actin cytoskeleton of cells was depolymerised and became more disordered. Apoptotic rate of cells (1.85%, 1.99%, and 2.29%) increased with the increase of n-HA concentration (5, 15, and 25 mu g/mL) and become significantly higher than the control. Total intracellular protein content decreased with n-HA concentration increase, showing significant difference between 25 mu g/mL and the control, and no significant change of ALP activity was observed at the 5th day. The results revealed that the cell growth was inhibited by n-HA in a concentration-dependent manner, and the obvious biological effects of MG-63 cells on n-HA existed at the low concentration range from 5 to 25 mu g/mL.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000296804600001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Nanoscience & NanotechnologyMaterials Science, MultidisciplinarySCI(E)EI0ARTICLEnull201