Add to ORKGan improved method was utilized for constructing hybrid arrested lambda phage cDNA library by subtractive hybridization, in which magnetic particles were applied to separate monomers from hybrids, and candidate sscDNAs were enriched by subtracting two control mRNAs from one treated sscDNA. The results showed that this new approach had a efficient improvement in constructing cDNA library which increased the yield of monomers, simplified experimental procedures and enriched more candidate genes.Biochemistry & Molecular BiologyBiophysicsSCI(E)中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)04373-3762
Rapid Amplification of cDNA Ends (RACE) is a technique used in molecular biology to obtain full leng...
Background: The construction of cDNA libraries is a useful tool to understand gene expression in org...
A new and highly effective method, termed suppression subtractive hybridization (SSH), has been deve...
This paper describes the construction and characterization of a family of λ phage cDNA cloning vecto...
We have developed a fast and efficient procedure for generating cDNA libraries in plasmid or phage l...
We describe the construction and use of two classes of cDNA cloning vectors. The first class compris...
Moderately induced genes often escape detection in conventional subtraction hybridisation cloning. H...
A subtractive hybridization method is described that allows the generation of a subtractive gene lib...
We describe a new method, called enzymatic degrading subtraction (EDS), for the construction of subt...
We have developed a cDNA library screening method which allows the simultaneous screening of>1012...
We describe the construction and characterization of two lambda surface displayed cDNA expression li...
We have developed a highly efficient method for the construction of a plasmid-based cDNA library. Th...
A procedure for the construction of general cDNA libraries is described which is based on the amplif...
Most cDNA enrichment techniques involve technically complicated subtractive and hybridization reacti...
In this study we present an improved polymerase chain reaction (PCR)-based methodology to generate l...
Rapid Amplification of cDNA Ends (RACE) is a technique used in molecular biology to obtain full leng...
Background: The construction of cDNA libraries is a useful tool to understand gene expression in org...
A new and highly effective method, termed suppression subtractive hybridization (SSH), has been deve...
This paper describes the construction and characterization of a family of λ phage cDNA cloning vecto...
We have developed a fast and efficient procedure for generating cDNA libraries in plasmid or phage l...
We describe the construction and use of two classes of cDNA cloning vectors. The first class compris...
Moderately induced genes often escape detection in conventional subtraction hybridisation cloning. H...
A subtractive hybridization method is described that allows the generation of a subtractive gene lib...
We describe a new method, called enzymatic degrading subtraction (EDS), for the construction of subt...
We have developed a cDNA library screening method which allows the simultaneous screening of>1012...
We describe the construction and characterization of two lambda surface displayed cDNA expression li...
We have developed a highly efficient method for the construction of a plasmid-based cDNA library. Th...
A procedure for the construction of general cDNA libraries is described which is based on the amplif...
Most cDNA enrichment techniques involve technically complicated subtractive and hybridization reacti...
In this study we present an improved polymerase chain reaction (PCR)-based methodology to generate l...
Rapid Amplification of cDNA Ends (RACE) is a technique used in molecular biology to obtain full leng...
Background: The construction of cDNA libraries is a useful tool to understand gene expression in org...
A new and highly effective method, termed suppression subtractive hybridization (SSH), has been deve...