A new rapid fluorometric method for determination of sulfonamides

A new fluorometric method for the determination of sulfonamides is described, based on the formation of a fluorescent inclusion complex of carbonic anhydrase(CA) with dansylamide (DNSA). The fluorescence maximum emission of the complex is at 460nm with excitation at 280nm. Dissociation constants were determined for the CA-sulfonamide complexes. Limits of detection for DNSA,sulfanilamide(SAN) and p-toluenesulfonamide (PTSN) were 0.84, 19.5 and 6.1 ng/ml, respectively. The recommended method, was applied. to the determination of sulfonamides in cow milk at ng/ml level.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000169109100014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Chemistry, MultidisciplinarySCI(E)0ARTICLE6495-496