Biotransformation of 2 alpha,5 alpha,10 beta,14 beta-tetraacetoxy-4(20), 11-taxadiene by cell suspension cultures of Catharanthus roseus

Catharanthus roseus cell suspension cultures were employed for the biotransformation of 2alpha,5alpha,10beta,14beta-tetraacetoxy-4(20),11-taxadiene (1), and four metabolites were obtained. Based on their physical and chemical data, the structures of the four metabolites were respectively elucidated as 10beta-hydroxy-2alpha,5alpha,14beta-triacetoxy-4(20),11-taxadiene (2), 5alpha-hydroxy-2alpha,10beta,14beta-triacetoxy-4(20),11-taxadiene (3), 6alpha,10beta-dihydroxy-2alpha,5alpha,14beta-triacetoxy-4(20),11-taxadiene (4), and 6alpha,9alpha,10beta-trihydroxy-2alpha,5alpha 14beta-triacetoxy-4(20),11-taxadiene (5), among which 3 and 5 were characterized as new taxoids. The effects of the stages of substrate addition on the biotrans formation were also investigated. The results revealed that the biotransformation rate for 1 reached 85.3% and the yield of 2 70% when 1 was administered during the mid-linear phase (9 - 12(th) day) of the cell growth cycle. On the other hand, the yield for 4 reached the highest level of 11.8% when 1 was added in the early linear phase (6(th) day).http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000180439100013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Plant SciencesChemistry, MedicinalPharmacology & PharmacySCI(E)PubMed13ARTICLE121113-11176