Nuclear magnetic resonance studies on Huwentoxin-XI from the Chinese bird spider Ornithoctonus huwena: N-15 Labeling and sequence-specific H-1, N-15 nuclear magnetic resonance assignments
- Peng, Kuan
- Lin, Ying
- Liang, Song-Ping
DOIAbstract
Huwentoxin-XI purified from the Chinese bird spider Ornithoctonus huwena is a toxin with both antiprotease activity and potassium channel blocking activity. To determine its solution structure, huwentoxin-XI was expressed in a yeast eukaryotic expression system and studied by NMR. The N-15 labeling strategy was used to facilitate the process of resonance assignments. The nearly complete sequence-specific assignments of proton and nitrogen resonances were obtained by analyzing a series of two-dimensional (2D) and three-dimensional (3D) spectra, including DQF-COSY, TOCSY, NOESY, N-15-H-1 HSQC, N-15-H-1 HNHA, N-15-H-1 HNHB, N-15-H-1 TOCSY-HSQC and N-15-H-1 NOESY-HSQC spectra. Secondary structure analysis of huwentoxin-XI showed that it mainly ...
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