Study of Inactivating Sulfate Reducing Bacteria with Zero-valent Iron Nanoparticles

在实验条件下通过混合振荡的方式,探究了零价铁纳米颗粒（NZVI）对硫酸盐还原菌（SRB）的杀灭能力和杀灭效果;并通过电镜扫描等手段观察零价纳米铁颗粒与硫酸还原菌形态变化,推测杀灭机制.结果表明,当向油田回注水中分别加入0、1、2、5和10mg/mL的零价铁时,该纳米粒子对硫酸还原菌的杀灭效率分别为0、75.6%、90.0%、99.9%和99.9%.染色与电镜结果说明,在杀灭过程中NZVI与SRB之间发生了紧密的吸附,并在细菌细胞表面发生了氧化作用.5mg/mL的NZVI对SRB有很强的杀灭作用,其作用机制主要是通过包被细胞、抑制细胞增殖,而非破坏细胞结构而达到.This research investigates the ability and effect of nanoscale zero-valent iron (NZVI) particles in inactivating sulfate-reducing bacteria; and conjectures the mechanism through observing transformation of SRB and NZVI. According to the research results, when adding 0, 1, 2, 5, and 10 mg/mL NZVI to the injection water, the inactivating rate of SRB would be 0, 75.6%, 90.0%, 99.9% and 99.9%. The dyeing and ESEM results show that NZVI adsorbs closely to the SRB cell when inactivating SRB, and it is oxidated on the surface of the bacteria cells. 5 mg/mL NZVI kills SRB greatly and the inhibition may mainly through coating the cells, inhibiting cell proliferation, rather than destroying the cellular structure.PubMed中文核心期刊要目总览(PKU)中国科技核心期刊(ISTIC)中国科学引文数据库(CSCD)0103040-30443