Posttreatment with Ma-Xing-Shi-Gan-Tang, a Chinese Medicine Formula, Ameliorates Lipopolysaccharide-Induced Lung Microvessel Hyperpermeability and Inflammatory Reaction in Rat

ObjectiveThe aim of present study was to investigate the efficacy of MXSGT, a traditional Chinese medicine formula used for treatment of respiratory system diseases, in the LPS-induced rat ALI particularly with a focus on its effect on lung microvascular hyperpermeability and inflammatory reaction. MethodsMale Sprague-Dawley rats were injected with LPS (7.5mg/kg, 1.5mg/mL) intraperitoneally. MXSGT (0.52g or 2.61g/kg) was given by gavage six hours after LPS injection. ResultsLPS stimulation resulted in a reduced survival rate, deteriorated vital signs, an increase in the number of leukocytes adhering to lung venules, the albumin leakage, the activity of MPO in lung tissues, the production of pro-inflammatory cytokines and lung perivascular edema. After LPS stimulation, western blot analysis revealed an increase in the expression of ICAM-1 and toll-like receptor 4, a decrease in tight junction proteins and an activation of cav-1, Src, and NF-B. All the LPS-induced alterations were significantly attenuated by posttreatment with MXSGT. ConclusionsThis study demonstrated MXSGT as a potential strategy for lung microvascular hyperpermeability and inflammatory reaction in ALI, and suggested that the beneficial role of MXSGT was correlated with toll-like receptor 4, Src, and NF-kappa B.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000343818400009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701HematologyPeripheral Vascular DiseaseSCI(E)PubMed3ARTICLEhanjingyan@bjmu.edu.cn7649-6632