Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis

To establish an in vitro model for the development of mouse spermatogenic cells into sperm by using the immunodefective mouse as the incubator.Tissue grafting was performed using testis from 1-2 days old Kun-ming mice as donor tissue and immunodefective mice as recipients; the expression of TESK1 mRNA in grafts was determined by RT-PCR and the spermatogenesis further observed with histological analysis of grafts.Molecular biological and histological analyses showed that grafts post-grafting not only expressed TESK1 mRNA as in normal mouse testis, but also exhibited similarities in the structure of seminiferous tubules and component of spermatogenic cells, including sperms.Spermatogenic cells heterotopically grafted in vitro could continuously grow and complete spermatogenesis and finally develop into sperm.PubMed06591-43