Investigations on gastric and colorectal cancers by FTIR spectroscopy

Thirty-one pairs of FTIR spectra of samples, including gastric and colorectal cancer and the corresponding normal tissues, were collected after surgery. FTIR spectra of cancerous tissue were significantly different from those of normal tissues. (1) Alterations of bands related to lipid: 2955, 2920, 2870, 2850 and 1740 cm-1 appeared less frequently in malignant tissue(P less than or equal 0.001). The ratio of I1460/I1400 in malignant tissue decreased significantly (P less than or equal 0.001), indicating that the relative quantity of lipid in malignant tissue decreased significantly. (2) Alterations of bands related to protein: in malignant tissue, the band of N-H and O-H shifted to a lower wave number (P = 0.025), suggesting that the degree of hydrogen-bonding of N-H and O-H increased. In malignant tissue, the ratios of I3375/I1460, I1640/I1460, I1550/I1460 raised (P less than or equal 0.01), respectively. These changes illustrate that the relative quantity of protein to lipid in malignant tissue increased significantly. The ratio of HW1550/I1550 increased obviously(P = 0.036), while HW1550 decreased significantly (P = 0.05), compared with those of normal tissue. We can conclude that the secondary structure of protein in malignant tissue changed. (3) Alterations of bands related to carbohydrate: in malignant tissue, the ratio of I1160/I1460 reduced (P =0.002). It might be due to the lessen of glycoprotein on the surface of malignant tissue, reversely, the ratio of I1120/I1460 raised(P = 0.019). The possible reason was that the glycogen grains on the surface of the malignant tissue increased. (4) Alterations of band related to nucleic acid: in normal tissue, the peak of P = 0 antisymmetric vibration was at (1241.61 ?? 6.15) cm-1. In malignant tissue, the band shifted toward a higher wave number (P = 0.033) due to the decreased hydrogen-bonding of P = O. FTIR spectroscopy could be a powerful tool for cancer diagnosis.EI03445-4492