G protein beta 3 subunit C825T polymorphism and essential hypertension in Chinese

To study the relationship between G-protein beta 3 subunit (GNB3) C825T variant and plasma renin-angiotensin system (RAS) activity in Chinese essential hypertensive patients.Case-control method was used. For 408 essential hypertensive patients who were enrolled in the trial groups, we chose 140 normotensives as control group 1 and 61 health persons with hypertensive familial history as control group 2, respectively. PCR-RFLP method was used to measure the C/T polymorphism. The results were observed by agarose gel electrophoresis.The frequencies of 825T allele were 45.6% to 56.4% in the three groups. Neither GNB3 genotype distribution nor the frequency of T allele was associated with essential hypertension. But patients with TT genotype had higher aldosterone level and lower angiotensin converting enzyme (ACE) activity than patients with CC genotype.In Chinese, patients with TT genotype had higher aldosterone level and lower angiotensin converting enzyme (ACE) activity.PubMed04423-53