Cytotoxic effects of sodium dodecyl benzene sulfonate on human keratinocytes are not associated with proinflammatory cytokines expression

Background Keratinocytes play a crucial role in the biological function of skin barrier. The relationship between sodium lauryl sulfate (SLS) and keratinocytes has been studied. However, the cytotoxicity and effects of sodium dodecyl benzene sulfonate (SOBS), a common detergent similar to SLS, on keratinocytes are still not known. This study aimed to investigate the effects of SDBS on cytotoxicity and expression of proinflammatory cytokines in cultured human keratinocytes. Methods This study was carried out using the keratinocytes cell line, HaCaT cells. The cytotoxicity of SDBS on HaCaT cells was evaluated with cell counting kit-8 (CCK-8) and phase-contrast microscopy. After exposure to different concentrations of SDBS, the total RNA of the HaCaT cells was extracted for evaluating the relative mRNA expression of IL-1 alpha, IL-6, IL-8, and TNF-alpha by qPCR. The supernatants of cells were collected for measuring the levels of IL-6 and IL-8 by enzyme-linked immunosorbent assay (ELISA). Results SDBS at concentrations of 20 mu g/ml and over showed direct cytotoxicity and induced morphological changes of the HaCaT cells. The mRNA expressions of IL-1 alpha, IL-6, IL-8, and TNF-alpha in different concentrations of SOBS at different time were comparable with that of controls. SDBS at concentrations of 5, 10, and 15 pg/ml had no significant effects on IL-6 and IL-8 excretion from HaCaT cells after,24-hour exposure. Moreover, no significant effects on the IL-6 and IL-8 excretion were found after 10 and 15 mu g/ml SDBS stimulations for 6, 12, and 24 hours, respectively. Conclusion SDBS at higher concentrations had cytotoxicity on HaCaT cells but had no effects on the mRNA expression of IL-1 alpha, IL-6, IL-8, and TNF-alpha, that was different from SLS.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000346329300018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Medicine, General & InternalSCI(E)PubMed中国科技核心期刊(ISTIC)中国科学引文数据库(CSCD)0ARTICLErmzjz@126.com213777-378112