Holographic coherent anti-Stokes Raman scattering bio-imaging

CARS holography captures both the amplitude and the phase of a complex anti-Stokes field, and can perform three-dimensional imaging by digitally focusing onto different depths inside a specimen. The application of CARS holography for bio-imaging is demonstrated. It is shown that holographic CARS imaging of sub-cellular components in live HeLa cells can be achieved. (C) 2012 Optical Society of AmericaBiochemical Research MethodsOpticsRadiology, Nuclear Medicine & Medical ImagingSCI(E)EI0ARTICLE71744-1749