Determination of papaverine by biotin-avidin amplified ELISA

Papaverine [1-(3,4-dimethoxybenzyl)-6,7-dimethoxyisoquinoline; PAP] is one of the most important alkaloids among the naturally occurring opium alkaloids. This work developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) for PAP based on the biotin-avidin amplification system. The linear range of the calibration curve and the limit of detection (defined as three times of the blank) were 0.02-1000 ng/mL and 0.008 ng/mL, respectively. The established method was successfully applied to determining the concentration of PAP in the pericarpium papaveris with the recoveries between 88% and 120%. It was proved to be a highly sensitive and practical method for trace PAP detection compared with the existing gas chromatographymass spectrometry (GC-MS) or high-performance liquid chromatography (HPLC) methods.Chemistry, AnalyticalSCI(E)7ARTICLE142977-29893