Frequent epigenetic silencing of protocadherin 10 by methylation in multiple haematologic malignancies

Ying, Jianming
Gao, Zifen
Li, Hongyu
Srivastava, Gopesh
Murray, Paul G.
Goh, Hwee Koon
Lim, Chai Yen
Wang, Yajun
Marafioti, Teresa
Mason, David Y.
Ambinder, Richard F.
Chan, Anthony T. C.
Tao, Qian

Open link

Publication date

January 2007

DOI

10.1111/j.1365-2141.2007.06512.x

Publisher

british journal of haematology

Journal

issn:0007-1048

Abstract

Epigenetic silencing of tumour suppressor genes (TSG) inactivates TSG functions. Previously, we identified PCDH10 as a methylated TSG in carcinomas. Here, we detected its frequent silencing and methylation in lymphoma cell lines including 100% Burkitt, 100% diffuse large B cell, 86% Hodgkin, 100% nasal natural killer/T-cell lymphoma and 1/3 of leukaemia cell lines, and in primary tumours but not in normal peripheral blood mononuclear cells or lymph nodes. PCDH10 silencing could be reversed by demethylation with 5-aza-2'-deoxycytidine. Methylation was further detected in 14% of Hodgkin lymphoma sera. Thus, PCDH10 methylation is frequently involved in lymphomagenesis and could serve as a tumour-specific biomarker.http://gateway.webofknow...