Effects of Lanthanide Ions(La3+ , Gd3+ , Yb3+ ) on Production of Reactive Oxygen Species in Mitochondria

研究了稀土离子对分离的线粒体产生活性氧(ROS)的影响. 采用荧光光度法跟踪线粒体内H2O2生成的动力学, 发现三种稀土离子(La3+, Gd3+, Yb3+)均能降低线粒体H2O2的生成;用黄嘌呤-黄嘌呤氧化酶体系进一步证明稀土对超氧阴离子(·O-2)存在清除作用, 而对H2O2无清除作用;测定了稀土对线粒体ROS代谢酶(谷胱甘肽过氧化物酶和超氧化物歧化酶)的活性影响. 结果表明, 三种稀土离子对线粒体谷胱甘肽过氧化物酶的活性基本没有影响, 而Gd3+和Yb3+稀土离子能明显抑制线粒体超氧化物歧化酶的活性.The effects of lanthanides (Ln) on the production of reactive oxygen species(ROS) in isolated mitochondria were investigated by fluorescence assays upon incubation with Ln(3+). The results show that Ln(3+) (La3+, Gd3+, Yb3+) could reduce the rates of overall ROS production. The results obtained from experiments on xanthine/xanthine oxidative enzyme system suggest that Ln(3+) ions can reduce the formation of O-center dot(2)-, but H2O2 did not react with Ln(3+). The influences of Ln(3+) on the activities of metabolize enzymes (GSH-Px and Mn-SOD) indicate that Ln(3+) could decrease the activity of Mn-SOD, but could not affect the activity of GSH-Px. The effects of Ln(3+) on cellular H2O2 level are discussed.国家自然科学基金http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000238377500003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701SCI(E)中文核心期刊要目总览(PKU)中国科技核心期刊(ISTIC)中国科学引文数据库(CSCD)106999-10022