Sensitive Determination of Saponins in Radix et Rhizoma Notoginseng by Charged Aerosol Detector Coupled with HPLC

With continuous development in analytical instruments over recent years, high performance liquid chromatography ( HPLC) with various detectors has become very important tools for routine analysis and quality control of TCMs and botanical medicines. As a new "mass'' detector, charged aerosol detector ( CAD) provides an alternative detection method, which ultraviolet (UV) is unable to achieve for components owning almost no chromophore in its chemical structures with the lower sensitivity. With the purpose of evaluating the applicability of CAD for the analysis of TCMs, an HPLC-CAD method for simultaneous determination of saponins in Radix et Rhizoma Notoginseng ("Sanqi'' in chinese) was established in this contribution, which was successfully applied for quantitation of seven saponins, notoginsenoside R(1), ginsenosides Rg(1), Re, Rb(1), Rg(2), Rh(1), and Rd, in thirty batches of Sanqi samples. Meanwhile, the LODs and LOQs of UV, ELSD and CAD were compared and the results showed that the CAD method exhibited a lower LOD ( 0.01 similar to 0.15 mu g) and LOQ ( 0.04 similar to 0.41 mu g) than UV and ELSD. Furthermore, the CAD exhibited a steadier baseline in gradient elution compared with UV at 203 nm. Also, the HPLC-CAD method presents many apparent advantages of high sensitivity, steady baseline in gradient elution, easy operating, and it was expected to be a sensitive and universal method for analysis of TCMs containing weak UV absorption compounds. It was demonstrated that the content of seven saponins in Radix et Rhizoma Notoginseng did not significantly correlate with the original work nor with the conventional quality standard.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000261875000006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Biochemical Research MethodsChemistry, AnalyticalSCI(E)EI18ARTICLE2242-2603