A Facile Method To Encapsulate Proteins in Silica Nanoparticles: Encapsulated Green Fluorescent Protein as a Robust Fluorescence Probe
- Cao, Aoneng
- Ye, Zhangmei
- Cai, Zhengwei
- Dong, Erya
- Yang, Xiaowei
- Liu, Gangbo
- Deng, Xiaoyong
- Wang, Yanli
- Yang, Sheng-Tao
- Wang, Haifang
- Wu, Minghong
- Liu, Yuanfang
Publication date
January 2010
DOI Publisher
angewandte chemie international edition ISSN
1433-7851 Journal
1433-7851 Citation count (estimate)
47Abstract
(Figure Presented) Si'l vous plait? A facile and general method has been developed to encapsulate polyhistidine-tagged proteins in silica nanoparticles (NPs; gray, see picture) using calcium ions (yellow). The enhanced green fluorescence protein (EGFP) encapsulated in the silica NPs shows a substantial increase in fluorescence intensity and stability against denaturants, protease, and heat. ? 2010 Wiley-VCH Verlag GmbH & Co. KGaA.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000277092900009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Chemistry, MultidisciplinarySCI(E)EI26ARTICLE173022-30254
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