The mechanism of stem cell factor in bone marrow stem cell transplantation to heart

目的探讨干细胞因子(SCF)在骨髓干细胞心肌内移植中的作用机制及对移植治疗的影响.方法对急性心肌梗死(AMI)大鼠进行骨髓干细胞心肌内移植治疗,RT-PCR方法检测骨髓中SCF表达水平的变化,并用SCF表达水平不同的骨髓干细胞进行心肌内移植治疗,超声心动图检测心脏结构、功能的变化;ELISA方法检测心肌组织、血和骨髓中SCF蛋白水平的变化.结果 AMI 1周时骨髓中SCF mRNA表达明显降低(P<0.01),接受移植治疗大鼠骨髓中SCF mRNA表达未出现降低.不同SCF表达水平的骨髓干细胞进行心肌内移植治疗均能使心脏射血分数达到70%,各组之间差异无统计学意义.移植治疗后1周血清中SCF蛋白的表达水平明显低于正常对照(P<0.05).结论骨髓干细胞心肌内移植可能通过SCF产生骨髓动员作用;心肌梗死或曾接受移植治疗个体的骨髓干细胞同样可用于心肌内移植治疗,不影响心功能的改善.To study the mechanism of stem cell factor (SCF) in bone marrow stem cells heart transplantation (BMT) and the influence of bone marrow mobilization on the transplantation efficacy.Rats with acute myocardial infarction (AMI) accepted BMT. The SCF expression in the bone marrow was measured by RT-PCR after the operation. Then bone marrow stem cells with different SCF levels for the transplantation were used and the cardiac function was compared by using echocardiography. The SCF protein expression in the heart, plasma and bone marrow was detected by ELISA.SCF expression level decreased significantly 1 week after AMI (P < 0.01), but it didn't decrease in those accepting BMT. Though the rats accepted BMT with bone marrow stem cells from different sources, the cardiac function showed no difference (P > 0.05). After BMT, the SCF protein level in the plasma decreased significantly (P < 0.05).BMT may make mobilization through SCF. Bone marrow stem cells from rats with AMI and also those with myocardial infarction plus BMT therapy can also be used for the transplantation into heart, and have no influence on cardiac function improvement.国家自然科学基金PubMed中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)010927-9313