A Selective Fluorescent Probe for Carbon Monoxide Imaging in Living Cells

A genetically encoded fluorescent probe is capable of selectively detecting carbon monoxide inside living cell. The probe, named COSer (CO sensor), consists of a circularly permuted yellow fluorescent protein (cpYFP) inserted into the regulatory domain of the bacterial CO-sensing protein, CooA, which gives the probe its selective CO-binding property. Copyright ? 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Chemistry, MultidisciplinarySCI(E)EI0ARTICLE389652-96565