Biotransformation of 2 alpha,5 alpha,10 beta,14 beta-tetra-acetoxy-4(20), 11-taxadiene by Ginkgo cell suspension cultures

Ginkgo biloba cell suspension cultures were used to biotransform 2 alpha ,5 alpha ,10 beta ,14 beta -tetra-acetoxy-4(20),11-taxadiene. Two novel compounds were obtained and their structures were identified as 9 alpha -hydroxyl-2 alpha ,5 alpha ,10 beta ,14 beta -tetra-acetoxy-4(20), 11-taxadiene 1 and 9 alpha, 10 beta -dihydroxyl-2 alpha ,5 alpha ,14 beta -tri-acetoxy-4(20), 11-taxadiene 2, respectively, on the basis of their physical and chemical data. Compound 1 was subsequently used as a substrate for the bioconversion by Ginkgo cell cultures, and the product obtained was confirmed to be the same as 2, which suggested that 2 is biosynthesized From 1. investigation on properties of the related enzymes responsible for the biotransformation reaction through the experimental techniques of cell-free culture and substrate/product concentration analysis revealed that the enzymes were extracellular and constitutive. (C) 2001 Elsevier Science Ltd. All rights reserved.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000169541300021&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Chemistry, OrganicSCI(E)23ARTICLE284677-46794