Microdetermination of proteins by resonance light scattering spectroscopy with bromophenol blue

Reaction of bromophenol blue with proteins results in an enhanced resonance light scattering at 334 nm. Based on this, a new quantitative determination method for proteins in the aqueous solution is established. This assay is characterized by high sensitivity (0.34-18.7 mu g/ml), short reaction time (>2 min), and simplicity (a one-step assay). Due to protein-to-protein variability, this method gave results higher than that of the bromocresol green assay in detection of human serum albumin. (C) 1996 Academic Press, Inc.Biochemical Research MethodsBiochemistry & Molecular BiologyChemistry, AnalyticalSCI(E)PubMed83ARTICLE186-9123