Preparative isolation and purification of acteoside and 2 '-acetyl acteoside from Cistanches salsa (C.A. Mey.) G. Beck by highspeed counter-current chromatography

High-speed counter-current chromatography (HSCCC) was applied to the separation and purification of phenylethanoid glycosides (PhGs) acteoside and 2 ' -acetylacteoside from Cistanches salsa (C.A. Mey) G. Beck with a quaternary two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (4:0.6:0.6:5, v/v). HPLC analyses of the CCC fractions revealed that the two main PhGs were over 98% purity. Their chemical structures were identified by LH NMR, C-13 NMR and MS. (C) 2001 Elsevier Science B.V. All rights reserved.Biochemical Research MethodsChemistry, AnalyticalSCI(E)PubMed1ARTICLE1181-18591