Synthesis and bioactivities of two multiple antigen peptides as potential vaccine against schistosoma

Based on the two antigenic peptides, 26-43 (P-26) and 116-131 (PI 16), derived from 28 kDa glutathione S-transferase of Schistosoma mansoni (Sm28GST), two multiple antigenic peptides (MAPs), (P-26)(4)-MAP and (P-116)(4)-MAP with the same oligomeric lysine core, were synthesized by stepwise solid-phase peptide synthesis method. The antigenicities and protective effects of these two MAPs were examined on experimental animals. As shown in the dot-ELISA result, the synthetic MAPs could be recognized and bound by immunoglobins in both patient's and infected-rabbit's sera. After Kunming mice were immunized with (P-26)(4)-MAP, the worm burden reduction rate and the liver egg reduction rate were 59.9% and 61.1%. In (P-26)(4)-MAP or (P-116)(4)-MAP immunized BALB/c mice, the worm burden reduction rates were 37.5% and 62.5%, respectively, and the liver egg reduction rates were 35.1% and 54.0%, respectively. (c) 2005 Elsevier Ltd. All rights reserved.Chemistry, MedicinalChemistry, OrganicSCI(E)PubMed7ARTICLE92415-24191