Substrate specificity for the 12 beta-hydroxylation of bufadienolides by Alternaria alternata

Hydroxylation is an important route to synthesize more hydrophilic compounds of pharmaceutical significance. Microbial hydroxylation offers advantages over chemical means for its high specificity. In this study, a fungal strain Alternaria alternata AS 3.4578 was found to be able to catalyze the specific 12 beta-hydroxylation of a variety of cytotoxic bufadienolides. Cinobufagin and resibufogenin could be completely metabolized by A. alternata to generate their 12 beta-hydroxylated products in high yields (> 90%) within 8h of incubation. A. alternata could also convert 3-epi-desacetylcinobufagin into 3-epi-12 beta-hydroxyl desacetylcinobufagin as the major product (70% yield). C-3 dehydrogenated products were detected in these reactions in fair yields, while their accumulation was relatively slow. The 12p-hydroxylation of bufadienolides could be significantly inhibited by the substitution of 1 beta-, 5-, or 16 alpha-hydroxyl groups, and the 14 beta,15 beta-epoxy ring appeared to be a necessary structural requirement for the specificity. For the biotransformation of bufalin, a 14 beta-OH bufadienolide, this reaction was not specific, and accompanied by 7 beta-hydroxylation as a parallel and competing metabolic route. The biotransformation products were identified by comparison with authentic samples or tentatively characterized by high-performance liquid chromatography-diode array detection-atmospheric pressure chemical ionization-mass spectrometry analyses. (c) 2005 Elsevier B.V. All rights reserved.Biotechnology & Applied MicrobiologySCI(E)EIPubMed0ARTICLE3253-26211