Determination of morphine by capillary zone electrophoresis immunoassay combined with laser-induced fluorescence detection

A competitive immunoassay for detecting morphine in bio-samples was established by capillary zone electrophoresis combined with laser-induced fluorescence detection (CZE-LIF); The antigen of morphine was labeled with isothiocyano-fluorescein (FITC) and then incubated with morphine monoclonal antibody and samples. The linear range was 50-1000 ng/mL, which was suitable for clinical and forensic, applications. The detection limit can reach 40 ng/mL, based on S/N = 2. The. recoveries of morphine from serum were satisfactory.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000220152400005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Biochemical Research MethodsImmunologyMedical Laboratory TechnologySCI(E)PubMed15ARTICLE157-702