Pig pancreatic alpha-amylase (PPA), an enzyme belonging to the alpha-amylase family, is involved in the degradation of starch. Like some other members of this family, PPA requires chloride to reach maximum activity levels. To further explain the mechanism of chloride activation, a crystal of wild-type PPA soaked with maltopentaose using a chloride-free buffer was analyzed by X-ray crystallography. A conspicuous reorientation of the acid/base catalyst Glu233 residue was found to occur. The structural results, along with kinetic data, show that the acid/base catalyst is maintained in the active site, in an optimum position, pointing toward the scissile bond-atom, due to the presence of chloride ions. The present study therefore explains the m...
Studies in Salt Activation. Part II. Influence of Salts on the Stability of Amylase
Mammalian alpha -amylases catalyze the hydrolysis of cr-linked glucose polymers according to a compl...
This article reports a first contribution for the elucidation of catalytic mechanism of Lipase from ...
ABSTRACT: Human pancreatic R-amylase (HPA) is a member of the R-amylase family involved in the degra...
To further investigate the mechanism and function of allosteric activation by chloride in some alpha...
Chloride is the allosteric effector of vertebrate pancreatic and salivary alpha-amylases and of the ...
The structural X-ray map of a pig pancreatic alpha-amylase crystal soaked (and flash-frozen) with a ...
The X-ray structure analysis of a crystal of pig pancreatic alpha-amylase soaked with a rho-nitrophe...
Human pancreatic α-amylase (HPA) is known to hydrolyze maltooligosaccharides with retention of anom...
In this work, we studied the catalytic mechanism of human pancreatic α-amylase (HPA). Our goal was t...
The structure of pig pancreatic alpha-amylase has been determined by X-ray diffraction analysis usin...
AbstractBackground: The intestinally located pancreatic enzyme, bile salt activated lipase (BAL), po...
The hydrolytic activity of P-amylase from Bacillus cereus var. mycoides was lost on replacement of e...
The alpha-amylase sequences contained in databanks were screened for the presence of amino acid resi...
Amylases catalyze the hydrolysis of starch material and play central roles in carbohydrate metabolis...
Studies in Salt Activation. Part II. Influence of Salts on the Stability of Amylase
Mammalian alpha -amylases catalyze the hydrolysis of cr-linked glucose polymers according to a compl...
This article reports a first contribution for the elucidation of catalytic mechanism of Lipase from ...
ABSTRACT: Human pancreatic R-amylase (HPA) is a member of the R-amylase family involved in the degra...
To further investigate the mechanism and function of allosteric activation by chloride in some alpha...
Chloride is the allosteric effector of vertebrate pancreatic and salivary alpha-amylases and of the ...
The structural X-ray map of a pig pancreatic alpha-amylase crystal soaked (and flash-frozen) with a ...
The X-ray structure analysis of a crystal of pig pancreatic alpha-amylase soaked with a rho-nitrophe...
Human pancreatic α-amylase (HPA) is known to hydrolyze maltooligosaccharides with retention of anom...
In this work, we studied the catalytic mechanism of human pancreatic α-amylase (HPA). Our goal was t...
The structure of pig pancreatic alpha-amylase has been determined by X-ray diffraction analysis usin...
AbstractBackground: The intestinally located pancreatic enzyme, bile salt activated lipase (BAL), po...
The hydrolytic activity of P-amylase from Bacillus cereus var. mycoides was lost on replacement of e...
The alpha-amylase sequences contained in databanks were screened for the presence of amino acid resi...
Amylases catalyze the hydrolysis of starch material and play central roles in carbohydrate metabolis...
Studies in Salt Activation. Part II. Influence of Salts on the Stability of Amylase
Mammalian alpha -amylases catalyze the hydrolysis of cr-linked glucose polymers according to a compl...
This article reports a first contribution for the elucidation of catalytic mechanism of Lipase from ...