Novel Infrared Spectroscopy Methods for Clinical Diagnosis of Tumor

报道了作者近年来利用红外光谱法检测肿瘤组织的阶段性研究进展.在比较研究了不同红外光谱方法的基础上,发现衰减全反射红外光谱法的检测结果与病理检验结果符合很好.该方法可直接测量冰冻和手术切除的新鲜离体肿瘤组织,测试简便,符合快速诊断的要求.另外,作者将中红外光纤和衰减全反射光谱测量方法相结合,实现了手术过程中原位、在体和实时检测肿瘤组织.累计研究了包括腮腺、颌下腺、食道、胃、肠、肝、胆囊、肾、乳腺、肺、甲状腺等十几种肿瘤和相应的正常组织.与病理诊断结果进行对照,红外光谱法诊断符合率在90%以上,表明该方法的普适性和准确性.In this paper, the authors have reviewed their investigation on the clinical detection of tumor tissues by infrared spectroscopy in recent ten years. Based on the comparison of different IR spectroscopic methods such as IR transmission spectroscopy, micro-IR spectroscopy etc, the authors found the good consistency of the results of ATR (attenuated total reglection) IR spectroscopic method with those of pathological biopsy. The authors have directly measured the IR spectra of frozen tissues stored in liquid nitrogen and freshly resected tissues, and have realized the measurement of tumor tissues in vivo during the operation process using a specially designed IR spectrometer connected with a mid-IR fiberoptic with an ATR probe. The authors have investigated the malignant and normal tissues including parotid, esophagus, stomach, colon, liver, gallbladder, breast, thyroid etc. and compared with the pathological results. The accuracy of this novel IR detection method is more than 90%.中国科学院资助项目SCI(E)EIPubMed中文核心期刊要目总览(PKU)中国科技核心期刊(ISTIC)中国科学引文数据库(CSCD)0111775-17782