A protein microarray prepared with phage-displayed antibody clones

Bi, Qun
Cen, Xiaodong
Wang, Wenjuan
Zhao, Xinsheng
Wang, Xuan
Shen, Ti
Zhu, Shenggeng

Open link

Publication date

January 2007

DOI

10.1016/j.bios.2007.02.011

Publisher

生物传感器与生物电子学

ISSN

0956-5663

Journal

issn:0956-5663

Citation count (estimate)

Abstract

Using a large phage antibody library, a protein microarray spotted directly with phage-displayed antibody clones was created to discriminate between recognition profiles of samples from healthy donors and leukemia patients. The protocol for preparing antibody-displaying phage chips was presented. Some conditions such as substrates and blocking buffers were compared and optimized. The major improvements of this microarray are higher throughput and lower cost compared to previous antibody chips. Due to its convenience and sensitivity, it can be extensively used for rapid and high throughput detection of protein profiles of experimental and clinical samples. (c) 2007 Elsevier B.V. All rights reserved.BiophysicsBiotechnology & Applied Micro...