HPLC-CAD in optimizating of saponins extraction from Radix et Rhizoma Notoginseng

目的:优选三七药材中皂苷类成分的提取工艺.方法:以三七皂苷R_1、人参皂苷Rg_1,Re,Rb_1,Rg_2,Rh_1和Rd的峰面积为指标,采用HPLC-CAD检测,对药材提取过程中粉碎粒度、提取溶剂、提取方法以及浸泡时间4个影响因素进行单因素考察,并对不同的浓度的甲醇、提取时间、提取次数以及提取溶剂用量进行L_9(3~4)正交试验设计分析.结果:三七药材的最优化提取工艺为,药材粉碎后过80目筛,室温浸泡0.5 h后,甲醇回流提取1次,提取时间为1.5 h,溶剂用量为每克20 mL.结论:优选的提取工艺操作简便、三七总皂苷提取率高,为三七皂苷提取工艺的进一步优化提供基础数据.To establish an optimized method for extracting saponins from Radix et Rhizoma Notoginseng (Sanqi).HPLC coupled with charged aerosol detector (CAD) was used for saponins determination. Peak areas of the main saponins notoginsenoside R1, ginsenosides Rg1, Re, Rb1, Rg2, Rh1 and Rd in Sanqi were monitored to evaluate the extract effectiveness. One-factor analysis was conducted to investigate the factors of particle size, solvent, extraction method and soaking time. Orthogonal experiment design L9 (3(4)) with different level of concentration of solvents, extraction time, extraction times and volume of the solvent, was implemented to determine the optimized extract condition.The optimum extract technique was that the dried and well pulverized (passed through a 80 mesh (180 +/-7.6) microm sample 1.0 g was extracted in 20 mL methanol by reflux (1.5 h) after soaked at room temperature for 0.5 h.The optimized technique is simple and high extraction efficiency. It provide basic data for further study on Sanqi.国家杰出青年科学基金PubMed中文核心期刊要目总览(PKU)中国科技核心期刊(ISTIC)中国科学引文数据库(CSCD)06677-6803