Study on microRNA expression in endometrium of luteal phase and its relationship with infertility of endometriosis

目的 探讨健康妇女黄体中期子宫内膜组织中微小RNA——mir-29c、mir-200a、mir-145的表达变化及其与内异症不孕的相关性.方法 选择2011年8月至2013年2月于北京大学深圳医院因不孕症行体外受精-胚胎移植助孕治疗、并已行宫腹腔镜检查、病理证实为内异症并排除其他不孕因素的患者36例作为内异症组,按照1985年美国生育学会修订的内异症分期标准,分为Ⅰ～Ⅱ期17例、Ⅲ～Ⅳ期19例;选择同期因男方因素不孕、女方完全正常的44例作为对照组.应用实时荧光定量PCR技术检测两组妇女黄体中期子宫内膜组织中mir-29c、mir-200a、mir-145的表达量;同时对内异症组辅助生殖技术治疗后妊娠与否进行随访.结果 (1)两组妇女子宫内膜组织中微小RNA的表达:内异症组患者子宫内膜组织中mir-29c、mir-200a、mir-145的平均表达量分别为2.46±1.98、3.20±2.45、6.378±3.275,对照组妇女分别为1.36±1.05、2.04±1.16、4.548±1.885,两组分别比较,差异均有统计学意义(P值分别=0.026、0.027、0.041).(2)不同期别内异症患者子宫内膜组织中微小RNA的表达:Ⅰ～Ⅱ期内异症患者子宫内膜组织中mir-29e、mir-200a、mir-145的平均表达量分别为0.53±0.51、0.33±0.26、0.048±0.021,Ⅲ～Ⅳ期内异症患者分别为0.26±0.18、0.28±0.12、0.045±0.016,两者分别比较,差异均无统计学意义(P值分别=0.191、0.661、0.753).(3)内异症组妊娠与否患者子宫内膜组织中微小RNA的表达:妊娠者子宫内膜组织中mir-29c、mir-200a、mir-145的平均表达量分别为0.60±0.30、1.23±0.48、0.886±0.238,未妊娠者分别为2.64±1.73、4.39±2.58、7.199±3.945,分别比较,差异均有统计学意义(P值分别=0.030、0.039、0.028).结论 内异症不孕患者黄体中期子宫内膜组织中mir-29c、mir-200a、mir-145表达上调,可能在内异症不孕中发挥作用.To study the different expression of microRNA(miRNA) including mir-29c, mir-200a, mir-145 in the mid-secretary endometrium and its relationship with infertility of endometriosis.From August 2011 to February 2013, 36 infertile cases with endometriosis confirmed by laparoscopy and pathology and excluded the other infertile factors in Department of Reproductive Medicine in Peking University Shenzhen Hospital were enrolled in this study, which were divided into 17 cases with stage I-II, 19 cases with stage III-IV according to the revised classification American Fertility Society. Forty-four healthy women with male factor infertility were chosen as control group. The relative expression levels of mir-29c, mir-200a, mir-145 in the endometrium of women in two groups were detected by using real-time quantitative polymerase chain reaction. Those women were followed up for pregnancy outcome of endometriosis group after assisted reproductive techniques (pregnancy and non-pregnancy group respectively).(1) The expression of miRNA between endometriosis and control groups:the average expression level of mir-29c, mir-200a, mir-145 in the endometrium of endometriosis group was 2.46 ± 1.98, 3.20 ± 2.45, 6.378 ± 3.275, which were significantly higher than 1.36 ± 1.05, 2.04 ± 1.16, 4.548 ± 1.885 in control group (P = 0.026, 0.027, 0.041, respectively). (2) The expression of miRNA between I-II stage and III-IV: the average expression level of mir-29c, mir-200a, mir-145 in the endometrium was 0.53 ± 0.51, 0.33 ± 0.26, 0.048 ± 0.021 in patients with of stage I-II, while 0.26 ± 0.18, 0.28 ± 0.12, 0.045 ± 0.016 in stage III-IV respectively, there were no statistically significant differences between the two groups (P = 0.191,0.661,0.753, respectively) . (3) The expression of miRNA between pregnancy and non-pregnancy groups:the average expression level of mir-29c, mir-200a, mir-145 in the endometrium were 0.60 ± 0.30, 1.23 ± 0.48, 0.886 ± 0.238 in pregnancy group, while 2.64 ± 1.73, 4.39 ± 2.58, 7.199 ± 3.945 in non pregnancy group, there were statistically significant differences between the two groups (P = 0.030,0.039,0.028, respectively).Up-regulation of mir-29c, mir-200a, mir-145 in the endometrial tissue might play a role in endometriosis associated infertility.2011年深圳市科技计划项目PubMed中文核心期刊要目总览(PKU)中国科技核心期刊(ISTIC)中国科学引文数据库(CSCD)012907-9104