Long-chain fatty acid oxidation changes in a beta 2 glycoprotein I-induced preeclampsia-like mouse model

Introduction: Abnormal fatty acid oxidation (FAO) and lipid metabolism have been found related to preeclampsia (PE). Antiphospholipid syndrome (APS) as a clinical risk factor for PE has also been reported with abnormal lipid metabolism. However, the role of FAO in PE accompanied with APS is unknown. We aimed to investigate long-chain FAO changes in a PE-like rodent model induced by beta 2-glycoprotein I (beta 2GPI). Methods: The PE-like model was established by injection of beta 2GPI (beta 2GPI group) or normal saline (control group) into C57BL/6J mice which were sacrificed on day 14 or 18 of gestation. Serum levels of anti-cardiolipin antibodies (aCL), anti-beta 2GPI antibodies (a beta 2GPI) and serum lipids were assayed. Lipid deposition in the placenta and maternal liver was detected by lipid staining. Long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) mRNA and protein expression in the placenta and maternal liver was analyzed. Results: The beta 2GPI group showed PE-like symptoms including hypertension, proteinuria and adverse pregnancy outcomes. Serum aCL, a beta 2GPI, free fatty acid (FFA) and triglyceride (TG) levels in the beta 2GPI group were significantly elevated compared with the corresponding control group (P < 0.05), while cholesterol showed no significant changes. Placenta and maternal liver fatty infiltration was found in the beta 2GPI group. LCHAD mRNA and protein expression in the placenta and maternal liver in the beta 2GPI group were significantly elevated compared with the corresponding control group (P < 0.05). Conclusion: beta 2GPI can induce PE-like symptoms, elevated serum FFA and TG, and abnormal LCHAD expression in pregnant mice. Changes in long-chain FAO could be a factor linking PE and APS. (C) 2014 Elsevier Ltd. All rights reserved.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000337659400009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Developmental BiologyObstetrics & GynecologyReproductive BiologySCI(E)PubMed7ARTICLEzi_yang@email.com6392-3973