Genetically encoded alkenyl-pyrrolysine analogues for thiol-ene reaction mediated site-specific protein labeling

A series of alkene-bearing pyrrolysine analogues were synthesized and subsequently incorporated into proteins at two sites by a mutant PylRS-tRNA pair with excellent efficiency. This strategy allowed the site-specific labeling of proteins carrying single or double genetically encoded alkene handles via bioorthogonal thiol-ene ligation reactions.Chemistry, MultidisciplinarySCI(E)EI0ARTICLE92766-2770