Study on the association of estrogen receptor genotypes with bone mineral density in Chinese postmenopausal Han women in Beijing

目的:研究雌激素受体(estrogen receptor,ER)基因多态性在中国北京地区绝经后的汉族妇女中的分布及其与骨密度的相关性.方法:采用聚合酶链式反应-限制性片段长度多态性(PCR-restriction fragment length polymorphisms,PCR-RFLP)方法研究ER基因的Xba Ⅰ和Pvu Ⅱ酶切多态性,检测骨密度,通过方差分析探讨ER基因的多态性分布与骨密度的关系.结果:ER基因Pvu Ⅱ酶切多态性分布与桡骨松质骨以及桡骨密质骨的骨密度之间不存在相关性,而ER基因Xba Ⅰ酶切多态性分布与桡骨松质骨以及桡骨密质骨的骨密度之间存在相关性,XX基因型骨密度值最低,xx基因型骨密度值最高.结论:ER基因Xba Ⅰ酶切多态性分布与桡骨松质骨以及桡骨密质骨的骨密度之间显著相关(P<0.05).本研究为探讨骨质疏松症在分子生物学范畴的发病机制及预防与预后提供了有益的依据.Objective:To investigate the distribution of polymorphism of estrogen receptor (ER) gene in postmenopausal Han women in Beijing as well as its relationship with bone mineral density (BMD).Methods:Xba Ⅰ,and Pvu Ⅱ polymorphisms of ER gene were studied by polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and BMD were determined by DEXA (dual-energy X-ray absorptiometry).The relationship between BMD and polymorphism of ER gene was studied by variance analysis.Results:Pvu Ⅱ polymorphism of ER gene was not associated with BMD of spongy and compact bone of radius;while Xba Ⅰ polymorphism of ER gene was associated with BMD of spongy and compact bone of radius.The lowest BMD was found with XX genotype while the highest BMD was found with xx genotype.Conclusion:There is high correlation between of Xba Ⅰ polymorphism and BMD of spongy and compact bone of radius.Our study suggested some bases to explore the pathogenesis of osteoporosis and to prevent the development of osteoporosis.中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)06508-5113