Expression of placental glucose transporters in pregnant women with abnormal glycometabolism

目的 研究妊娠合并糖代谢异常孕妇胎盘内葡萄糖转运蛋白(glucose transporter,GLUTs)的表达变化,探讨其对母儿间葡萄糖转运功能的影响及其与新生儿体重的关系. 方法 于北京大学第一医院收集孕前糖尿病孕妇的胎盘组织标本7例、妊娠期糖尿病A110例、妊娠期糖尿病A214例,妊娠期糖耐量受损10例及正常孕妇胎盘组织标本15例,用免疫组织化学方法测定GLUT1和GLUT3的表达,并记录新生儿出生体重. 结果 免疫组化显示GLUT1主要表达于胎盘的细胞滋养细胞和合体滋养细胞,各组间GLUT1的表达强度差异具有统计学意义(P＜0.01);GLUT3在部分胎盘绒毛问质的血管内皮细胞中表达.各组间的表达差异也具有统计学意义(P＜0.01).GLUT1的表达强度与新生儿出生体重存在等级相关性(rs=0.532,P＜0.01),GLUT3表达强度则与新生儿出生体重无相关性(rs=0.178,P＞0.01). 结论 妊娠合并糖代谢异常孕妇胎盘内GLUT1和GLUT3的表达增强,且GLUT1在胎儿对葡萄糖的摄取过程中可能发挥主要作用.Objective To investigate the expression of glucose transporters(GLUTs)in placentas of pregnant women with abnormal glyeometabolism,and to explore its effect on glucose transport between mother and fetus and its relation with the birth weight.Methods Placentas of 41 pregnant women with abnormal glycometabolism(7 cases of DM,10 GDM A1,10 GIGT and 14 GDMA2)and 15 normal pregnant women as control were selected.The expression of GLUT1 and GLUT3was detected by immunohistochemistry.The birth weight was measured at delivery.Results GLUT1 was expressed in the syncytiotrophoblasts and cytotrophoblasts,whereas GLUT3 in some endothelial cells.The expressions of GLUT1 and GLUT3 were significantly different among the five groups(P＜0.01).Positive correlation was shown between the GLUT1 expression and the birth weight(rs=0.532,P＜0.01),but not in GLUT3 expression.Conclusions The expression of GLUT1 and GLUT3 in placentas of pregnancy with abnormal glycometabolism is enhanced,and GLUT1 may play a predominant role in the fetal glucose uptake.中国科技核心期刊(ISTIC)06373-3761