Expression of tumor necrosis factor-related apoptosis-inducing ligand receptors in renal cell carcinoma

目的研究肿瘤坏死因子相关诱导凋亡配体（TRAIL）受体在肾癌组织中的分布及其意义。方法采用RT-PCR及Northern Blot的方法检测TRAIL受体在肾癌组织及正常肾组织，肾癌细胞系GRC-I和正常肾小管细胞系HK-2中的表达。结果死亡受体DR4、DR5在肾癌组织及正常肾组织，肾癌细胞系GRC-I和正常肾小管细胞系HK-2中强表达；假受体DcR-1在正常肾组织和正常肾小管细胞系HK-2中强表达；假受体DcR-2未见表达。结论 TRAIL基因在肾癌肿瘤细胞的凋亡机制中可能发挥重要的作用。Objective To investigate the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptors in renal cell carcinorma. Methods TRAIL receptors mRNA were tested through RT-PCR and Northern Blot in renal cell carcinoma tissue and renal cell carcinoma cell line (GRC-I). Results The expression of death receptor 4(DR4) and death receptor 5(DR5) was certificated in renal cell carcinoma and normal renal cells. Decoy receptor 1(DcR1) expressed only in normal renal tissue and cell line. Decoy receptor 2(DcR2) was not detected in normal and tumor tissue and cell line. Conclusion TRAIL gene may play an important role in apoptosis of renal cell carcinoma.中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)05375-3773