Expression and significance of CD44 and angiogenic growth factor in nasal NK/T cells lymphoma

目的探讨鼻型自然杀伤(NK/T)细胞淋巴瘤高度侵袭性的分子机制。方法对17例鼻型NK/T细胞淋巴瘤病人的活检组织标本，采用免疫组织化学法检测CD20、CD43、CD45RO、CD3ε、CD44、CD56、细胞毒抗原TIA-1；原位杂交法检测血管内皮生长因子（VEGF）、碱性纤维母细胞生长因子（bFGF）、EB病毒（EBV）。结果 17例鼻型NK/T细胞淋巴瘤中，12例CD3ε阳性，15例CD43/CD45RO、CD56阳性，17例TIA-1阳性，CD20均阴性，7例CD44阳性，11例VEGF弱阳性或阳性，3例bFGF弱阳性，15例EBV阳性。3例肿瘤细胞异型性明显者CD44均为阴性，7例死亡者中4例CD44阴性；VEGF在血管浸润4例中有2例阴性，7例死亡者中4例阴性；bFGF 在血管浸润4例中2例阴性，7例死亡者中6例阴性。结论 CD44与鼻型NK/T细胞淋巴瘤的不良预后无明显相关性；VEGF在鼻型NK/T细胞淋巴瘤的表达明显高于bFGF，其生物学意义及临床预后意义有待进一步探讨。To investigate the molecular basis of the high aggressiveness of nasal NK/T cells lymphoma.Immunohistochemical study was used on 17 cases of nasal NK/T cells lymphoma to examine CD20, CD43/CD45RO, CD3, CD56, TIA-1, and CD44. In situ hybridization was used to examine Epstein-Barr virus (EBV) infection, VEGF, and bFGF.The positive rates of CD20, CD43/CD45RO, CD3, CD56, TIA-1, and CD44 were 0%, 88.2%, 70.6%, 88.2%, 100%, and 41.2%. Fifteen cases (88.2%) were positive in EBER1 (EBV infection). VEGF was expressed in 11 cases (64.7%). VEGF expression was negative in 2 out of 4 cases with angioinvasion, and negative in 4 cases out of 7 cases who died of tumor. bFGF was weakly expressed in 3 cases (17.6%) and was not expressed in 2 out of 4 cases with angioinvasion and 6 out of 7 cases who died of tumor. CD44 expression was negative in all 3 cases with dysplasia, and in 4 out of the 7 cases who died of tumor.CD44 can not predict the potentiality of NK/T cells lymphoma in invasion, and does not correlate with prognosis. The expression of VEGF is far more frequent than that of bFGF in NK/T cells lymphoma. The significance of VEGF and bFGF expression remains to be investigated.PubMed中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)013776-7788