Cell origin of localized Castlemnan's disease of hyaline-vascular type

目的从基因水平了解6例Castleman's病(CD)病理细胞组成特点.方法肿物切除以后,苏木精-伊红染色作常规组织病理检查以明确肿物的组织学类型;应用免疫组化法研究细胞表型,明确肿物的细胞组成;根据细胞组成,应用R-PCR和克隆测序方法了解肿物主要细胞克隆组成.结果6例患者皆为限局性透明血管型CD;肿瘤组织滤泡样结构中主要为B淋巴细胞,滤泡间隙为T淋巴细胞.RT-PCR扩增产物经聚丙烯酰胺凝胶电泳后得到单一条带,RT-PCR产物克隆测序得到128 bp、122 bp两种碱基长度的克隆,相同长度序列间具有高度一致性,克隆内分别存在12个和7个碱基的差异.结论6例患者肿瘤组织中含有单/寡克隆瘤性B细胞,这些B细胞起源于生发中心细胞.To investigate the cell features of 6 Castleman's disease patients and evaluate their prognosis.The resected tumors were investigated by routine histopathology and immunohistochemistry. Reverse-transcript PCR (RT-PCR) and sequencing of RT-PCR products were used to assess the clonal characters of the main tumor cells.Histologically, all of the 6 tumors were classified as the hyaline vascular type. B-cells dominated the follicular germinal centers, with T-cells dispersing inter-follicularly. The results of RT-PCR each obtained a single band of either 128 bp or 122 bp and sequencing showed that there was highly homogeneity within the same length sequences, accompanied by fewer different nucleotide acids.Monoclonal and/or oligoclonal B cells were identified in Castleman's disease. These B cells were originated from germinal center cells.PubMed中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)07397-4002