A Comparative Study of Malignant Tissue Diagnosis Using ATR and Microscopy FTIR Spectroscopy

在前期的肿瘤组织红外光谱诊断方法研究中, 采用水平衰减全反射(ATR)红外光谱诊断法在多种肿瘤组织样品中取得了与病理诊断很好符合的实验结果; 针对文献报道中多采用的显微红外光谱法, 文章对同一肿瘤组织样品应用两种方法采集红外光谱, 比较测量条件及光谱诊断结果, 研究结果表明ATR法主要测量了整块组织的光谱信息, 避免了组织结构不均一, 微区采样扫描取点少对光谱诊断结果的影响, 获得的光谱可以为临床应用提供判断依据.A comparative FTIR study was undertaken between ATR (attenuated total reflection) and infrared microsoopy on their application to measuring the same tissue for cancer diagnosis. The measurements showed that the coincidence of pathological diagnosis with ATR is higher than that of microscopy FTIR method. The result indicated that the measured area of tumor tissue is critical. The area for microscopy measurement is similar to250 mum(2), it reflects the heterogeneity of the tissue. However, the ATR measurement provides more information of malignant tissue for the measured area is 1.9 x 10(5) times than microscopy. Therefore, ATR exhibit the high accuracy in cancer detection for clinical application.国家重点基础研究发展计划(973计划); 国家自然科学基金; 高等学校博士学科点专项科研项目http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000223843100009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701SCI(E)PubMed中文核心期刊要目总览(PKU)中国科技核心期刊(ISTIC)中国科学引文数据库(CSCD)68930-9322